α7 nAChR, Novus Biologicals, NBP1-79948
What do reviewers generally think about NBP1-79948 from Novus Biologicals?
Researchers report mixed performance for this antibody in rat α7 nAChR studies. In one lot, Western blotting failed to distinguish transfected from untransfected GH4C1 cells. In another lot, it detected expected bands in α7 nAChR and α7-GFP cells, absent in controls, but produced multiple non-specific background bands. For fluorescent immunocytochemistry, 1:100 staining was similar in transfected and untransfected cells, while 1:1000 showed significant difference. One reviewer points out that dilution strongly affected immunostaining specificity. Overall, results are application- and lot-dependent, with concerns about background and inconsistent specificity.
Information about this antibody
Vendor notes
User Reviews (2)
Review of: α7 nAChR, Novus Biologicals, NBP1-79948 by Ralph H Loring, Department of Pharmaceutical Science, Northeastern University, Boston, Massachusetts
Western blotting results obtained with Lot No QC42753-42341 of this antibody did not show any significant differences between GH4C1 cells transfected with rat α7 nAChR and untransfected control cells.
For further information see: Evaluating Commercially Available Antibodies for Rat alpha7 Nicotinic Acetylcholine Receptors
Garg and Loring, Journal of Histochemistry and Cytochemistry 2017. Vol. 65(9) 499-512 https://www.ncbi.nlm.nih.gov/pubmed/28763248
0 useful
0 not usefulReview of: α7 nAChR, Novus Biologicals, NBP1-79948 by Ralph H Loring, Department of Pharmaceutical Science, Northeastern University, Boston, Massachusetts
This antibody was evaluated in a study of commercially available antibodies against rat alpha7 Nicotinic Acetylcholine Receptors (α7 nAChR). Seven antibodies were included in the study and tested in the applications Western blotting and fluorescent immunocytochemistry.
Both tests were performed using the rat pituitary cell line GH4C1, which has no endogenous expression of α7 nAChR. For immunofluorescence, antibody staining of native cells was compared with staining of cells transfected with rat α7 nAChR cDNA. Expression of α7 nAChR in GH4C1 was confirmed by RT-PCR. The same two cell-lines were used for Western blotting, together with GH4C1 cells transfected with C-terminally GFP tagged α7 nAChR or GFP.
This antibody (Lot No QC0391-42133) was tested in two different dilutions for immunostaining. Diluted 1:100 the antibody resulted in similar staining of transfected and untransfected cells, but a 1:1000 dilution of the antibody showed significant difference between the two cell lines. In Western blotting bands of expected size were detected in α7 nAChR and α7 nAChR-GFP cells. Both bands were absent in control cells. However, multiple non-specific background bands were detected in all cell lines.
For further information see: Evaluating Commercially Available Antibodies for Rat alpha7 Nicotinic Acetylcholine Receptors
Garg and Loring, Journal of Histochemistry and Cytochemistry 2017. Vol. 65(9) 499-512 https://www.ncbi.nlm.nih.gov/pubmed/28763248
Comments
0 useful 0 not useful
Comments