α2A Adrenergic Receptor, Santa Cruz Biotechnology, sc-1478

The specificity of this antibody was evaluated in immunohistochemistry of murine ureteres and stomach tissue, as well as western blotting of lysed CHO cells.

Immunohistochemisry showed highly distinct staining patterns in rat ureter and stomach.
However, western blotting of cells stably expressing the α2A receptor in CHO cells showed no difference in signal compared to lysates of cells with no endogenous expression. Thus we conclude that this antibody has no specificity for the target antigen.

Dilutions:
WB: 1:400
IHC: 1:100

Ref: Pradidarcheep et al 2009. Lack of specificity of commercially available antisera
against muscarinergic and adrenergic receptors

https://www.ncbi.nlm.nih.gov/pubmed/?term=19198807