α1D Adrenergic Receptor, Santa Cruz Biotechnology, sc-10721

This antibody was included in a test of ten commercially available antibodies against alpha-1-adrenergic receptor subtypes. All antibodies were tested using Western blotting of brain and heart tissue from WT mice and knock out controls with genetic deletion of one, two or all three alpha-1-adrenergic receptor subtypes.

None of the antibodies detected a band of the appropriate size, or a band of larger or smaller size, that was present in WT mouse heart or brain and absent in KO heart or brain.

For further information see BC Jensen et al., 2009 (https://link.springer.com/article/10.1007%2Fs00210-008-0368-6)

The specificity of this antibody was evaluated in immunohistochemistry of murine ureteres and stomach tissue, as well as western blotting of lysed CHO cells.

Immunohistochemisry showed highly distinct staining patterns in rat ureter and stomach.
However, western blotting of cells stably expressing the α1D receptor in CHO cells showed no difference in signal compared to lysates of cells with no endogenous expression. Thus we conclude that this antibody has no specificity for the target antigen.

Dilutions:
WB: 1:400
IHC: 1:50

Ref: Pradidarcheep et al 2009. Lack of specificity of commercially available antisera
against muscarinergic and adrenergic receptors

https://www.ncbi.nlm.nih.gov/pubmed/?term=19198807