AT1 receptor, Abcam, ab9391
What do reviewers generally think about ab9391 from Abcam?
Researchers report that AT1 receptor Abcam ab9391 is discontinued and performs poorly. One reviewer points out that Western blotting of hypothalamus, liver, and kidney cortex from wild-type and AT1A knockout mice showed no specific signal in wild-type tissues, and immunocytochemistry of transfected 4B cells also showed no specific signal versus mock controls. The overall consensus is that the antibody is non-specific for AT1 receptor detection in these applications.
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Review of: AT1 receptor, Abcam, ab9391 by admin
This antibody has been discontinued
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0 not usefulReview of: AT1 receptor, Abcam, ab9391 by Juan M. Saavedra, Georgetown University
The specificity of this antibody was evaluated by Western blot analysis of tissue homogenates from hypothalamus, liver and kidney cortex obtained from WT and AT1A receptor knock out mice. No specific signal was detected in WT tissues compared to control tissues.
In addition, the antibody was tested by immunocytochemistry staining of rat hypothalamic neuronal cell line 4B transfected with AT1A receptor. 4B cells have no endogenous expression of AT1 receptors, and control staining was performed using mock transfected cells. No specific signal was detected in transfected cells compared to control cells.
Ref: Benicky et al., 2012. Six Commercially Available Angiotensin II AT1 Receptor Antibodies are Non-specific
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508356/
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