ApoM, Sigma-Aldrich, WH0055937M1

This antibody was tested in a screening for antibodies suitable for ELISA-based detection of apoM in human plasma as described in Bosteen et al. 2015 (http://www.jlr.org/content/56/3/754.long).

Briefly, initial testing of 15 commercially available anti-human apoM mAbs was performed by dot blotting of bacterially expressed 6xHis tagged recombinant human apoM (0.9µM) and human plasma and Western blotting of human plasma.

ELISA screening was performed as a competitive ELISA using recombinant human apoM as bait (20 nM) and plasma apoM as competitor. For detection was used anti-6xHis-HRP antibody. The final sandwich ELISA was performed using DTT/iodoacetamide treated plasma samples and rabbit mAb anti-apoM clone EPR2904 as detection antibody.

The performance of tested antibodies is summarized in inserted table.