Immunoglobulins, DakoCytomation, P0448
What do reviewers generally think about P0448 from Dako?
Researchers report that Immunoglobulins, DakoCytomation, P0448 performs well as a secondary antibody in Western blotting. It is an HRP-conjugated goat anti-rabbit antibody used with short 1-hour incubations, at 1:2000 or 1:5000 dilution in milk-containing buffer, followed by brief washes and ECL detection. One reviewer points out that it can be diluted 1:2000 in TBST plus 5% milk. The reviews indicate reliable performance with no specific complaints.
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User Reviews (2)
Review of: Immunoglobulins, DakoCytomation, P0448 by Teresa Martín
Used as a secondary antibody in Western Blot. Incubation time: 1 hour.
Antibody was diluted 1:2000 in TBST + milk (5%).
0 useful
0 not usefulReview of: Immunoglobulins, DakoCytomation, P0448 by Heidi Gytz Olesen, McGill University, Canada
Goat anti-rabbit HRP conjugate, DakoCytomation
Short protocol:
1:5000 dilution
Used as secondary antibody for western. The membrane was incubated with the secondary antibody in 5% low fat milk powder in PBS-T for 1 h at RT on a shaker. 4×5 min washing steps were then carried out. To visualize the proteins, ECL+ Plus Western Blotting detection System (GE Healthcare) was used according to the manufacturers recommendations
Review: Worked very well
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