Gephyrin, Synaptic Systems, 147 021
What do reviewers generally think about 147 021 from Synaptic Systems?
Researchers report that Gephyrin, Synaptic Systems, 147 021 performs well for immunostaining and immunocytochemistry. It worked well at 1:2000 for fluorescence microscopy of perfusion-fixed mouse tissue and at 1:500-1:1000 for primary hippocampal neurons, where it specifically labeled inhibitory postsynaptic densities. One reviewer points out that the hybridoma supernatant did not show unspecific nuclear staining, unlike the purified mAb7a IgG discussed in a related review.
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Review of: Gephyrin, Synaptic Systems, 147 021 by David I. Hughes, University of Glasgow
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:2000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes
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0 not usefulReview of: Gephyrin, Synaptic Systems, 147 021 by Peter Lund Ovesen
Gephyrin, mouse mAb, mAb7a hybridoma supernatant, Synaptic Systems 147 021,
ICC (1:500-1:1000)
Great antibody, stains specificly inhibitory postsynaptic densities in primary hippocampal neruons. I didn’t observe any unspecific staining of the nucleus for the hybridoma supernatant as opposed to the mAb7a purified IgG (mentioned in my review of 147 108).
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