CD56, BD Bioscience, 345810
Information about this antibody
Target
Alt. Targets
Vendor
Catalog No
345810
Application
Clonality
mAb
Origin
Mouse
Reactivity
Human
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: CD56, BD Bioscience, 345810 by Kasper Mølgaard Jensen, Center for Cancer Immune Therapy (CCIT), Herlev Hospital, Denmark
Anti-human CD56, PE-conjugated, clone MY31, BD Bioscience #345810
By manufactures instruction this clone is not recommended for intracellular cytokine staining (ICS).
PE anti-CD56 was used in an antibody cocktail for ICS of PBMCs Expressing a chimeric antigen receptor.
Protocol in short: 3uL/sample added to antibody cocktail at a final volume of 20uL. 20uL antibody cocktail added to cells to a final volume of approximately 30uL. Cells incubated in dark for 20 minutes before washing, fixation, and intracellular staining.
Indeed, anti-CD56 clone MY31 did not work in ICS for identifying CD3-/CD56+ NK cells.
Comments