CD56, BD Bioscience, 345810

BD Bioscience 345810 · CD56 · mAb · Mouse
Applications: Flow Cytometry
2.23
(Overall rating from 1 review)
#2 of 5 targeting CD56
2.23
From 1 review · #2 of 5 for CD56

Information about this antibody

Target
Catalog No
345810
Application
Clonality
mAb
Origin
Mouse
Reactivity
Human
Conjugation
N/A
Product Link
N/A

Vendor notes

No vendor notes available for this antibody.

pAbmAbs Average Ratings

ℹ
Flow Cytometry
2.60
Overall
2.23

User Reviews (1)

Review of: CD56, BD Bioscience, 345810 by Kasper Mølgaard Jensen, Center for Cancer Immune Therapy (CCIT), Herlev Hospital, Denmark

Reviewed: October 29, 2017

Anti-human CD56, PE-conjugated, clone MY31, BD Bioscience #345810

By manufactures instruction this clone is not recommended for intracellular cytokine staining (ICS).
PE anti-CD56 was used in an antibody cocktail for ICS of PBMCs Expressing a chimeric antigen receptor.

Protocol in short: 3uL/sample added to antibody cocktail at a final volume of 20uL. 20uL antibody cocktail added to cells to a final volume of approximately 30uL. Cells incubated in dark for 20 minutes before washing, fixation, and intracellular staining.

Indeed, anti-CD56 clone MY31 did not work in ICS for identifying CD3-/CD56+ NK cells.

Flow Cytometry

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