Bak, Santa Cruz Biotechnology, sc-832
What do reviewers generally think about sc-832 from Santa Cruz Biotechnology?
Researchers report mixed performance for Bak sc-832. One reviewer points out that the Bak signal was weaker than aspecific signal, though specificity was confirmed in Bak KO cells using 1:1000 in 1% milk in PBS-T. Another notes unspecific bands in immunoblotting at 1:500 in 5% milk, but Bak was visible, and endogenous Bak could be immunoprecipitated from HEK293 cells with 2 μg antibody per 1.5 mg Protein G beads, albeit with very high background. The antibody is also reported discontinued.
Information about this antibody
Vendor notes
User Reviews (3)
Review of: Bak, Santa Cruz Biotechnology, sc-832 by Dario Rizzotto
Signal corresponding to Bak was weaker than aspecific signal. Specificity was confirmed on Bak KO cells. Dilution 1:1000 in 1% milk in PBS-T
For more information see Rizzotto et al. Cell Reports 2020, “Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30”. https://doi.org/10.1016/j.celrep.2020.03.011
0 useful
0 not usefulReview of: Bak, Santa Cruz Biotechnology, sc-832 by admin
This antibody has been discontinued
Comments
0 useful 0 not usefulReview of: Bak, Santa Cruz Biotechnology, sc-832 by Heidi Gytz Olesen, McGill University, Canada
Rabbit anti-Bak (G-23), Santa Cruz Biotechnology, Sc-832.
1:500 for immunoblotting
2 μg/1.5mg beads for IP (Dynabeads® Protein G kit)
Short protocol:
For immunoblotting I dilute in 5% low fat milk powder in PBS-T and incubate overnight at 4°C.
Dynabeads kits (Invitrogen) were used according to manufacturers recommendations.
Review:
Unspecific bands were present after immunoblotting, however the Bak protein itself was clearly visible as well. We were able to immuno precipitate endogenous Bak from HEK293 cells, though the background from the Bak antibody was very high.
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0 useful 0 not useful
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