OPA1, Abcam, Ab42364
Information about this antibody
Target
Alt. Targets
OPA1
Vendor
Catalog No
Ab42364
Application
Clonality
pAb
Origin
Rabbit
Reactivity
Human, Mouse, Rat
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: OPA1, Abcam, Ab42364 by Heidi Gytz Olesen, McGill University, Canada
Rabbit anti-Opa1, Abcam Ab42364
Short protocol:
1:2000 for immunoblotting
1.4 μg/1.5mg beads for IP (Dynabeads® Protein G kit)
For immunoblotting I diluted in 5% low fat milk powder in PBS-T and incubate overnight at 4oC.
Dynabeads kits (Invitrogen) for IP were used according to manufacturers recommendations.
Review: The antibody efficiently precipitated endogenous Opa1 from Hek293 cells, though excessive co-elution of the immunoglobulins were detected as well. Several unspecific bands were present upon immunoblotting, however the Opa1 protein was clearly noticeable.
0 useful
0 not useful
Comments