GAPDH, Cell Signaling Technology, 2118
What do reviewers generally think about 2118 from Cell Signaling Technology?
Researchers consistently report that this GAPDH antibody performs well for western blotting as a loading control, with strong, reliable signals in total lysates and cell lines. Reported dilutions range from 1:1000 to 1:5000, including use for reprobing membranes and mouse hippocampal tissue. One reviewer points out that the signal is strong enough to allow lower concentrations, using 1:1000 in TBST with 1% BSA after 1.5 hours incubation. No negative performance issues are mentioned.
Information about this antibody
Vendor notes
User Reviews (5)
Review of: GAPDH, Cell Signaling Technology, 2118 by Tiago Gil Oliveira, University of Minho
I have used this antibody for western blotting of total lysates from mice hippocampal tissue in a dilution of 1:3000. This antibody works so well for me, that I often use it as a control of my blotting protocol.
For more information see Santa-Marinha et al. Cell Reports 2020, “Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning”
https://www.sciencedirect.com/science/article/pii/S2211124720302837#sec4
0 useful
0 not usefulReview of: GAPDH, Cell Signaling Technology, 2118 by Vishal Gohil, Texas A&M University
Used for western blotting with great results.
Dilution used: 1:1000
For more information see Soma et al. Cell Reports 2019, “COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase”. https://www.sciencedirect.com/science/article/pii/S2211124719315360#bib15
Comments
0 useful 0 not usefulReview of: GAPDH, Cell Signaling Technology, 2118 by Giorgio Ramadori, University of Geneva, Department of Cellular Physiology and Metabolism
We have used this antibody for western blotting with great succes.
For more information see Aras et al., 2019, Cell Reports 27, 2385–2398 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30573-X)
Comments
0 useful 0 not usefulReview of: GAPDH, Cell Signaling Technology, 2118 by Teresa Martín
Used as a primary antibody in Western Blot. Amount of cells lysated for the WB varied from 1×106 to 2.5×106. Really strong signal, can be used in lower concentrations. Incubation 1.5 hrs.
Antibody was diluted 1:1000 in TBST + BSA (1%).
Comments
0 useful 0 not usefulReview of: GAPDH, Cell Signaling Technology, 2118 by Peter Lund Ovesen
GAPDH (14C10) Rabbit mAb #2118 Cell Signaling
Excellent antibody for the loading marker GAPDH. Used for reprobing of membranes at a dilution of 1:5000.
Tested on Human neuroblastoma cell line SH-SY5Y.
Size: 37 kDA
Comments
0 useful 0 not useful
Comments