Mas receptor, Santa Cruz Biotechnology, sc-135063
What do reviewers generally think about sc-135063 from Santa Cruz Biotechnology?
Overall, the reviews indicate the antibody is discontinued and has limited performance. One reviewer points out that lot K2311 gave an expected band just below 37 kDa in Mas-transfected HEK293 cells by Western blot at 1:1000, but with strong nonspecific bands in both transfected and mock cells, a weak signal, and no detection of endogenous Mas in mouse heart or kidney lysates; immunofluorescence was tested at 1:200.
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User Reviews (2)
Review of: Mas receptor, Santa Cruz Biotechnology, sc-135063 by admin
This antibody has been discontinued
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0 not usefulReview of: Mas receptor, Santa Cruz Biotechnology, sc-135063 by Marina Cecilia Muñoz, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires
This antibody (lot #K2311) produces a signal of expected size (just below the 37 kDa marker band) in HEK293 cells transiently transfected with Mas receptor compared to mock transfected cells. A number of unspecific bands (some very strong) were, however, observed in both cell lines.
It was not possible to detect a signal from endogenous Mas receptor in lysate from heart and kidney mouse tissue.
WB 1:1000
The antibody also gives a weak signal in Mas receptor transfected HEK293 cells compared to untransfected cells.
IF 1:200
Ref: Burghi et al., PLoS One. 2017 Aug 16;12(8):e0183278, a table summary of antibodies tested in the study is inserted
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