CD3, BD Bioscience, 345764

Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.

a total of 2,5uL used in a 20uL mastermix with CD4, CD8 and CD56 used to stain approx. 300.000 cells.

Anti-human CD3 (FITC conjugated), clone SK7, BD Bioscience, #345764

Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.

Works very well for detection of lymphocytes in flow cytometry. The antibody was diluted in a master mix as 2.5uL anti-CD3 antibody for 20uL total volume per sample.