CD3, BD Bioscience, 345764
What do reviewers generally think about 345764 from BD Bioscience?
Researchers agree that CD3, BD Bioscience, 345764 performs well for detecting lymphocytes in flow cytometry during intracellular cytokine secretion assays. Both used the FITC-conjugated antibody in a master mix with CD4, CD8, and CD56, adding 2.5 uL to 20 uL total volume per sample. One reviewer points out that titrations and compensation had already been performed in-lab, and the panel stained about 300,000 cells. No performance complaints are mentioned.
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User Reviews (2)
Review of: CD3, BD Bioscience, 345764 by Arianna Draghi
Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.
a total of 2,5uL used in a 20uL mastermix with CD4, CD8 and CD56 used to stain approx. 300.000 cells.
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0 not usefulReview of: CD3, BD Bioscience, 345764 by Kasper Mølgaard Jensen
Anti-human CD3 (FITC conjugated), clone SK7, BD Bioscience, #345764
Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection of lymphocytes in flow cytometry. The antibody was diluted in a master mix as 2.5uL anti-CD3 antibody for 20uL total volume per sample.
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