CD4, BioLegend, 317432
What do reviewers generally think about 317432 from BioLegend?
Researchers report that CD4, BioLegend, 317432 works well in flow cytometry for intracellular cytokine staining and phenotype gating of T-cell subpopulations. Both used it in a master mix for ICS. One reviewer points out that 1.7 uL was used in a 20 uL master mix for about 300,000 cells, with titration and compensation performed previously. No complaints are mentioned.
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User Reviews (2)
Review of: CD4, BioLegend, 317432 by Arianna Draghi
Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.
a total of 1,7uL used in a 20uL mastermix with CD3, CD8 and CD56 used to stain approx. 300.000 cells.
0 useful
0 not usefulReview of: CD4, BioLegend, 317432 by Kasper Mølgaard Jensen
Anti-human CD4 (PerCP conjugated), clone OKT4, Biolegend, #317432
Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection of lymphocytes subpopulations in flow cytometry. The antibody was diluted in a master mix as 5.0uL anti-CD4 antibody for 20uL total volume per sample.
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