Estrogen receptor beta, Dako, M7292
Information about this antibody
Target
Vendor
Catalog No
M7292
Application
N/A
Clonality
mAb
Origin
Mouse
Reactivity
N/A
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: Estrogen receptor beta, Dako, M7292 by Cecilia Williams, KTH Royal Institute of Technology, Sweden
Andersson et al. Nature Communications 2017 describe the validation of thirteen commercial and academic antibodies raised against estrogen receptor beta (ERbeta).
The present antibody failed in the IHC validation step (1:60 dilution) since it recognized both ERbeta positive cells and ERbeta negative cells.
In Western blotting it did not work. The antibody was also unable to pull down ERbeta by immunoprecipitation (IP) as determined by mass spectrometry, but pulled down numerous other nuclear proteins.
The antibody is also provided by Thermofisher and BioRad.
See data figure and link to full article above.
Comments