CD271, BD Bioscience, 557196
What do reviewers generally think about 557196 from BD Bioscience?
The reviews report that CD271, BD Bioscience, 557196 performs well for detecting human NGFR/CD271 in electroporated tumor-infiltrating lymphocytes and PBMCs by FACS staining. Staining with 20 uL in 0.5 mL FACS buffer for 30 minutes at 4°C gave high specific signal for NGFR-expressing cells and no signal in negative controls. One reviewer points out that 10 uL can stain about 500,000 cells in 200 uL FACS buffer for 30 minutes at 4°C in the dark and works just as well as 20 uL.
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Review of: CD271, BD Bioscience, 557196 by Kasper Mølgaard Jensen, Center for Cancer Immune Therapy (CCIT), Herlev Hospital, Denmark
Tumor Infiltraing Lymphocytes electroporated with truncated Nerve Growth Factor Receptor (tNGFR) and detected with PE mouse anti-human NGFR (CD271) after 24 and 48h.
Previously used 20uL to stain around 500.000 cells in 200uL FACS buffer.
Here I used 10uL to stain around 500.000 cells in 200uL (30 minutes at 4 degrees, dark) and it works just as good.
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0 not usefulReview of: CD271, BD Bioscience, 557196 by Kasper Mølgaard Jensen
PE Mouse Anti-Human CD271, BD Bioscience, #557196 (clone C40-1457)
PE-conjugated monoclonal antibody specific for the human Nerve Growth Factor Receptor (NGFR).
Used to stain human peripheral blood mononuclear cells (PBMCs) electroporated with NGFR mRNA.
PBMCs were stained with 20uL PE-anti-CD271 in a total volume of 0.5mL FACS buffer (PBS+5% Human serum) for 30 minutes at 4 degrees.
High specific signal was observed for NGFR expressing PBMCs with no signal from negative control samples.
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