IFN-gamma, Mabtech, 3420-3
Information about this antibody
Target
Alt. Targets
Vendor
Catalog No
3420-3
Application
Clonality
mAb
Origin
Mouse
Reactivity
Human
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: IFN-gamma, Mabtech, 3420-3 by Kasper Mølgaard Jensen
Anti-human IFN-gamma mAb, clone: 1-D1K, MABTECH, #3420-3
mAb used as coating antibody in ELISpot assay to detect cellular immune response of chimeric antigen receptor modified T-cells against CD19 positive B-cells.
Anti-hIFNy was diluted in PBS to a final concentration of [7.5 ug/mL] (coating volume: 75uL) (incubation ON at RT)
Used in combination with biotinylated anti-human IFNy detection antibody (clone: 7-b6-1).
Plate was washed between each step using 6 x 200uL PBS (or PBS + 0.05% tween).
Very good results were obtained with clear individual spots, a clear dose-dependent response and low background. Positive control: staphylococcal enterotoxin B [2ug/mL].
0 useful
0 not useful
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