IFN-gamma|Interferon-gamma, Mabtech, 3420-6
Information about this antibody
Alt. Targets
Vendor
Catalog No
3420-6
Application
Clonality
mAb
Origin
Mouse
Reactivity
Human, Non-Human Primate
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: IFN-gamma|Interferon-gamma, Mabtech, 3420-6 by Kasper Mølgaard Jensen
Biotinylated anti-human IFN-gamma mAb, clone: 7-B6-1, MABTECH, #3420-6
mAb used as detection antibody in ELISpot assay to detect cellular immune response of chimeric antigen receptor modified T-cells against CD19 positive B-cells.
Biotinylated anti-hIFNy was diluted in PBS supplemented with 1% BSA and 0.02% NaN3 to a final concentration of [0.75 ug/mL] (final volume: 75uL) (incubation two hours at RT)
Plate was washed between each step using 6 x 200uL PBS (or PBS + 0.05% tween).
Very good results were obtained with clear individual spots, a clear dose-dependent response and low background. Positive control: staphylococcal enterotoxin B [2ug/mL].
0 useful
0 not useful
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