GI Syn, Santa Cruz Biotechnology, sc-6640
What do reviewers generally think about sc-6640 from Santa Cruz Biotechnology?
Researchers report GI Syn sc-6640 is discontinued. In one use, it was applied at 1:200 (1 µg/mL) to 10 µm mouse and rat dorsal root ganglia sections and gave clear specific satellite glial cell staining. One reviewer points out that rat sections showed some unspecific nuclear staining in neurons, which was not observed in mouse sections. Overall, the antibody appears useful for detecting GI Syn in satellite glial cells, with a caveat about species-dependent nuclear background in rat.
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Review of: GI Syn, Santa Cruz Biotechnology, sc-6640 by admin
This antibody has been discontinued
0 useful
0 not usefulReview of: GI Syn, Santa Cruz Biotechnology, sc-6640 by Sara Buskbjerg Jager, Department of Biomedicine, Aarhus University
GI Syn antibody (C-20), sc-6640, Santa Cruz.
Goat anti-glutamine synthetase antibody.
The antibody was used 1:200 (1 ug/mL) on sections of mouse and rat dorsal root ganglia.
The dorsal root ganglia had beed fixed for 1.5 hrs (for mouse) or 4 hrs (for rat) in PFA. Cryoprotected overnight in 30% sucrose and finally embeded in tissue tek. The sections were cut in 10 um sections.
The antibody gave a clear specific staining of the satellite glial cells. However, in the rat sections there was some unspecific staining in the nucleus of the neurons. This was not seen on the mouse sections.
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