p-RSK p90 (phospho T359/S363), Abcam, ab32413
Information about this antibody
Alt. Targets
Vendor
Catalog No
ab32413
Application
Clonality
mAb
Origin
Rabbit
Reactivity
Human, Mouse, Rat
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (2)
Review of: p-RSK p90 (phospho T359/S363), Abcam, ab32413 by Anders Dalby
This antibody works very well with clear bands and almost no background signal.
Dilution: 1:1000
Review of: p-RSK p90 (phospho T359/S363), Abcam, ab32413 by Simon Mølgaard Jensen, Department of Biomedicine, Aarhus University
p-RSK ab32413 (formerly know as MBL int. polyclonal antibody, AT-7163)
I have tested this on lysates from mouse hippocampal neurons with great results!
The neurons were stimulated with 1nM BDNF for 10 minutes before lysis in lysis-buffer containing phos-stop and complete.
50ug protein was loaded per sample. The antibody was used at a 1:1000 dilution and left to incubate over night at 4 degrees.
A clear signal was observed in the sample stimulated with BDNF while a very weak band was observed in the unstimulated sample.
Comments