Phospho-Src, Cell Signaling Technology, 2101
What do reviewers generally think about 2101 from Cell Signaling Technology?
Researchers report that Phospho-Src CST 2101 performs well for immunoblotting of tumor and sarcoma cell lysates, including after dasatinib treatment to assess Src inhibition. One reviewer points out that the manufacturer-recommended 5% BSA dilution worked, although they used a higher antibody concentration. Reported dilutions were 1:500 and 1:1000, with use in RIBA buffer lysates from PFSK-1 and D324 cells. No major complaints are mentioned beyond adjusting concentration.
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User Reviews (2)
Review of: Phospho-Src, Cell Signaling Technology, 2101 by Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University
This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
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0 not usefulReview of: Phospho-Src, Cell Signaling Technology, 2101 by Christina Demuth, Department of Clinical Medicine, Aarhus University
Phospho-Src (Tyr416), Cell signaling 2101 (rabbit)
The antibody was used on sarcoma cell lines after treatment with the Src-inhibitor dasatinib , to evaluate the effect. For this purpose the antibody was great. The manufacturer recommends diluting in 5 % BSA, that worked for us, though we increased the antibody concentration.
Dilution: 1:500
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