Phospho-mTOR, Cell Signaling Technology, 5536

Cell Signaling Technology 5536 · Phospho-mTOR · mAb · Rabbit
Applications: Western Blot, Flow Cytometry, Immunostaining, IP, ChIP
2.70
(Overall rating from 1 review) #2 of 2 targeting Phospho-mTOR
Overall Rating
2.70
From 1 review · #2 of 2 for Phospho-mTOR

Information about this antibody

Target
Phospho-mTOR
Alt. Targets
mTOR
Vendor
Cell Signaling Technology
Catalog No
5536
Application
Western Blot, Flow Cytometry, Immunostaining, IP, ChIP
Clonality
mAb
Origin
Rabbit
Reactivity
Human, Drosophila Melanogaster, Mouse, Rat, Monkey
Conjugation
N/A
Product Link
N/A

Vendor notes

No vendor notes available for this antibody.

pAbmAbs Average Ratings

ℹ
Western Blot
2.86
Overall
2.70

User Reviews (1)

Review of: Phospho-mTOR, Cell Signaling Technology, 5536 by lars meinertz byg, Department of Biomedicine, Aarhus University

Reviewed: January 30, 2017

Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536
I used this antibody on murine hippocampal neurons with or without stimulation of BDNF (this was confirmed using pERK-as surrogate). I did see a band in the stimulated lane not present in the control. This was however situated around 200 kDa, while the protein is 289 kDa. At the expected size there was a smeared band, which did appear to be upregulated in the stimulated lane, it was however not very clear.
Konc.: 1:1000
Exposure: 30 min
lane-load: 50 ug
Please see image above

Ratings from this reviewer
Western Blot

Comments

Lars Meinertz Byg31 reviews
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