CREB, Cell Signaling Technology, 9197

I have tested this antibody on mouse HepG2 cells.
50ug protein were loaded in each well for western blot.
Dilution: 1:1000
The membrane was left to incubate over night at 4 C in blocking buffer (milk).
The antibody did not work.

This antibody works well on mouse liver lysates to detect total CREB however does not work on primary murine neuronal lysates. Dilution 1:1000.

CREB (48H2), Rabbit mAb #9197, Cell Signaling Technologies

I have used this antibody for detection of total CREB without any valuable results.
Western blotting of murine hippocampal neurons in a dilution of 1:1000, as recommended by the supplier, did not yield any results. Reprobing the same blot with beta-actin proved that the blotting procedure was succesful.