CREB, Cell Signaling Technology, 9197
What do reviewers generally think about 9197 from Cell Signaling Technology?
Researchers report mixed performance for CREB CST 9197 in western blotting. It works well for total CREB in mouse liver lysates at 1:1000, but fails in primary murine neuronal and hippocampal neuron lysates. One reviewer points out that beta-actin reprobing confirmed successful blotting. Another reviewer found it did not work in mouse HepG2 cells at 1:1000 after overnight 4C incubation in milk. Overall, it appears useful for some mouse liver samples but unreliable for neuronal applications.
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User Reviews (3)
Review of: CREB, Cell Signaling Technology, 9197 by Michelle Pedersen
I have tested this antibody on mouse HepG2 cells.
50ug protein were loaded in each well for western blot.
Dilution: 1:1000
The membrane was left to incubate over night at 4 C in blocking buffer (milk).
The antibody did not work.
0 useful
0 not usefulReview of: CREB, Cell Signaling Technology, 9197 by Anders Dalby
This antibody works well on mouse liver lysates to detect total CREB however does not work on primary murine neuronal lysates. Dilution 1:1000.
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0 useful 0 not usefulReview of: CREB, Cell Signaling Technology, 9197 by Signe Petersen, Aarhus University
CREB (48H2), Rabbit mAb #9197, Cell Signaling Technologies
I have used this antibody for detection of total CREB without any valuable results.
Western blotting of murine hippocampal neurons in a dilution of 1:1000, as recommended by the supplier, did not yield any results. Reprobing the same blot with beta-actin proved that the blotting procedure was succesful.
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