SUR-1, Santa Cruz Biotechnology, sc-25683
What do reviewers generally think about sc-25683 from Santa Cruz Biotechnology?
Overall consensus is limited because one reviewer notes discontinuation, while another reports poor Western blot performance in rat brain: lot G0313 produced low molecular weight nonspecific bands and no expected 140-170 kDa SUR1 signal, making it invalid for that application. One reviewer points out that a replacement lot A2016 yielded a double band near 150 kDa and faint 280-300 kDa dimer, but identity was not confirmed by LC-MS/MS.
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User Reviews (2)
Review of: SUR-1, Santa Cruz Biotechnology, sc-25683 by admin
This antibody has been discontinued
0 useful
0 not usefulReview of: SUR-1, Santa Cruz Biotechnology, sc-25683 by Adam Munhall, Portland VA Medical Center
Anti SUR1 (ABCC8), polyclonal, 611-690, Santa Cruz Biotechnology, “H-80”, item sc-25683, lot G0313, 1:200, against 30ug (BSA method) rat brain homogenate lysed with RIPA buffer, blotted on low autofluorescence PVDF, illuminated with anti-mouse Dylight 800-4xPEG (Thermo, 1:200), scanned on NIR imager, showed numerous low MW non-specific bands, but nothing in the 140-170kDa range as expected for full length SUR1. Blocking was with 3% goat. Not valid for WB in rat brain.
NOTE: Santa Cruz provided a 2nd lot to try, A2016, and this provides a double band around 150kDa and faintly again around 280-300kDa (presumably dimer)– but have not been able to verify with LC-MS/MS as containing SUR1. Will update if a second SUR1 antibody can mimic this result.
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