Strep-tag, Chromeo, 2-1544-050
Information about this antibody
Target
Alt. Targets
Vendor
Catalog No
2-1544-050
Application
Clonality
mAb
Origin
Mouse
Reactivity
N/A
Conjugation
Chromeo 488
Product Link
N/A
Conjugated
Yes
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: Strep-tag, Chromeo, 2-1544-050 by Seandean Harwood, Aarhus University
StrepMab-Classic Chromeo 488 conjugate
Cat.no: 2-1544-050 from Iba
This fluorophore-antibody conjugate was used in a FACS for the detection of a StrepII-tagged protein binding to a protein expressed on the surface of HEK293 cells. Only a 2x shift in the MFI was obtained, compared to other detection systems (e.g. StrepMab classic combined with a GAM-PE) which gave 20-50x shifts in the FMI. The StrepMab Classic Clone generally works very well, in my experience, but I can’t say the same for this conjugate.
FACS: used 200x diluted in PBS with 1% FBS.
0 useful
0 not useful
Comments