PARP, Cell Signaling Technology, 9542
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User Reviews (3)
Review of: PARP, Cell Signaling Technology, 9542 by Dario Rizzotto
Good signal in western blot. Recognizes the full length and cleaved form of the protein. Dilution 1:1000 in 1% milk in PBS-T
For more information see Rizzotto et al. Cell Reports 2020, “Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30”. https://doi.org/10.1016/j.celrep.2020.03.011
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0 not usefulReview of: PARP, Cell Signaling Technology, 9542 by Heidi Gytz Olesen, McGill University, Canada
Rabbit anti-PARP, Cell Signalling #9542
Short protocol:
1:2000 dilution in 5% low fat milk powder in PBS-T and incubate overnight at 4°C.
Review: Continuously performs very well. I kept the diluted antibody at -20° and reused it up to 5 times. Run the gel appropriately to ensure complete separation of the larger cleaved fragment (89kDa) and uncleaved PARP (116kDa).
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0 useful 0 not usefulReview of: PARP, Cell Signaling Technology, 9542 by Paula Szalai, University of Oslo
PARP Cell Signaling Technology rabbit #9542
Works very well, strong specific signal. Used in cell lines (LNCaP, PC3)
Detects full-lenght at 116 kDa and cleaved at 89 kDa, apoptotic signal.
Use 1:1000 TBS Tween 5% BSA, can be reused often and also be refreshed when it is getting weak.
Please see image above
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