HLA Class I, Sigma-Aldrich, H1650
What do reviewers generally think about H1650 from Sigma-Aldrich?
Researchers report that HLA Class I, Sigma-Aldrich, H1650 (clone W6/32) works well for detecting human MHC class I using 5 µL per million cells and GAM-PE detection. It is used as a positive control, including on cell lines serving as negative controls, and gave very good staining of human CEA-positive MKN45 gastric carcinoma cells after 30 minutes. One reviewer points out that they switched from a directly PE-conjugated anti-MHC class I antibody because this format better matches their usual murine primary antibody followed by GAM-PE analysis.
Information about this antibody
Vendor notes
User Reviews (2)
Review of: HLA Class I, Sigma-Aldrich, H1650 by Seandean Harwood, Aarhus University
anti-HLA CLass I mAb, clone W6/32
Sigma
Used at 5 uL/million cells, detection with GAM-PE
This antibody is used at my lab to detect human MHC class I, as a positive control (especially on cell lines which are being used as negative controls for the experiment at hand). We switched to this from an anti-MHC Class I already directly conjugated to PE, as this one is more comparable to how our samples are typically analyzed (with an initial murine anti-whatever, followed by GAM-PE).
0 useful
0 not usefulReview of: HLA Class I, Sigma-Aldrich, H1650 by Kasper Mølgaard Jensen, Aarhus University
Monoclonal Anti-HLA Class I Antigen antibody produced in mouse, clone W6/32, H1650 Sigma-Aldrich
Used 5µL to stain 10^6 human CEA-positive MKN45 gastric carcinoma cells for 30 minutes prior analysis.
Very good results alone with GAM-PE pAb.
Comments
0 useful 0 not useful
Comments