IgG (Fc region), Jackson ImmunoReseach Europe, 115-116-071
What do reviewers generally think about 115-116-071 from Jackson ImmunoReseach Europe?
Researchers report consistent use of this goat anti-mouse IgG Fc fragment PE reagent in flow cytometry/FACS for sandwich detection with various mouse primaries. It works at about 1:200 in 1% FBS PBS for 10^6 cells and gives strong results, including ~50x MFI shifts for abundant surface antigens. One reviewer points out that it is widely used in their lab with anti-myc, anti-FLAG, and anti-StrepII primaries. No complaints are mentioned.
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Review of: IgG (Fc region), Jackson ImmunoReseach Europe, 115-116-071 by Seandean Harwood, Aarhus University
AffiniPure F(ab’)₂ Fragment Goat Anti-Mouse IgG (H+L) – phycoerythrin
Jackson Immunoresearch
This antibody is widely used at my laboratory in FACS for sandwich detection, using various mouse antibodies (e.g. anti-myc, anti-FLAG, anti-StrepII), and then detecting with this GAM-PE. We use it diluted 200x in 1% FBS PBS. When detecting abundant antigens (e.g. EGFR on HeLa cells, heterogenously expressed surface receptors in HEK293 cells under CMV promoter control), we get MFI shifts around 50x.
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0 not usefulReview of: IgG (Fc region), Jackson ImmunoReseach Europe, 115-116-071 by Kasper Mølgaard Jensen, Aarhus University
R-Phycoerythrin AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG, Fcy Fragment Specific, 115-116-071, Jackson ImmunoResearch
Used for flow cytometry analysis at working concentration of 1:200 for staining 10^6 cells.
Great results with a variety of different primary mAb.
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