IgG (Fc region), Jackson ImmunoReseach Europe, 115-116-071

AffiniPure F(ab’)₂ Fragment Goat Anti-Mouse IgG (H+L) – phycoerythrin
Jackson Immunoresearch

This antibody is widely used at my laboratory in FACS for sandwich detection, using various mouse antibodies (e.g. anti-myc, anti-FLAG, anti-StrepII), and then detecting with this GAM-PE. We use it diluted 200x in 1% FBS PBS. When detecting abundant antigens (e.g. EGFR on HeLa cells, heterogenously expressed surface receptors in HEK293 cells under CMV promoter control), we get MFI shifts around 50x.

R-Phycoerythrin AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG, Fcy Fragment Specific, 115-116-071, Jackson ImmunoResearch

Used for flow cytometry analysis at working concentration of 1:200 for staining 10^6 cells.
Great results with a variety of different primary mAb.