Immunoglobulins, Dako, P0260
What do reviewers generally think about P0260 from Dako?
Researchers generally report that this HRP-conjugated rabbit anti-mouse secondary antibody performs well for western blotting and ELISA. It was used at 1:1000 in ELISA and 1:2000 on neuron lysates for western blotting, with 1-hour incubations. One reviewer points out that it was diluted in a blocking buffer containing 1x TST, 2% milk, 2% Tween-20, and MiliQ water, and incubated at room temperature. No complaints are mentioned.
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Vendor notes
User Reviews (3)
Review of: Immunoglobulins, Dako, P0260 by Simon Glerup
Excellent HRP-conjugated secondary rabbit anti-mouse antibody for western blotting
0 useful
0 not usefulReview of: Immunoglobulins, Dako, P0260 by Teresa Martín
Used in an ELISA assay as the secondary antibody. Incubation time was 1 hour.
Antibody was diluted 1:1000.
Comments
0 useful 0 not usefulReview of: Immunoglobulins, Dako, P0260 by Signe Petersen, Aarhus University
Dako, Polyclonal Rabbit Anti-Mouse immunoglobulins, HRP.
P0260
This was used as a secondary antibody on neuron lysates in a dilution of 1:2000. The antibody was diluted in blocking buffer consisting of 1x TST buffer, 2% Milk, 2% Tween-20 and MiliQ H2O. It was incubated for 1 hour at RT.
I used this antibody for western blotting with excellent results.
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0 useful 0 not useful
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