GAD65, ImmuQuest, IQ442
What do reviewers generally think about IQ442 from ImmuQuest?
Researchers report GAD65 ImmuQuest IQ442 performs well in Western blotting on mouse hippocampal lysates and frozen tissue, producing a specific, clear band at the expected 65 kDa with no signal in liver. One reviewer points out that they used 1 ug/ml primary antibody overnight at 4°C and secondary antibody for 1 hour at room temperature, yielding a nice band. Overall consensus is positive for mouse tissue Western blotting, with no complaints mentioned.
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User Reviews (3)
Review of: GAD65, ImmuQuest, IQ442 by Peter Lund Ovesen
Mouse hippocampal lysates, 30 ug protein / well.
This Ab shows a specific band at the expected size of GAD2/GAD65 (65 kDa)
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0 not usefulReview of: GAD65, ImmuQuest, IQ442 by Abdel-Rahman Al-Absi
GAD65, MOUSE MAB, IMMUQUEST #IQ442
I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1ug/ml) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives nice band.
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0 useful 0 not usefulReview of: GAD65, ImmuQuest, IQ442 by Simon Mølgaard, Aarhus University
mouse monoclonal Gad65 (IQ442) from ImmuQuest.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 1ug/ml, so for this experiment i used a concentration of 1 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
This antibody is excellent for detecting GAD65 by western blotting! A clear band was observed in the hippocampus-sample while nothing was seen in the liver, as expected.
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