A-beta, BioLegend, 803001
What do reviewers generally think about 803001 from BioLegend?
Researchers report that A-beta, BioLegend, 803001 performs well for detecting amyloid beta in human tissue and hiPSC-derived cerebral organoids. One reviewer points out that it works fine for immunocytochemistry of hiPSC-derived cerebral organoids at 1:2000. Another reviewer used it at 1:500 in 1% BSA/PBS with ice-cold acetone fixation and DAKO EnVision secondary on human postmortem cortex cryosections, finding it worked beautifully and compared favorably with clone 4G8 for amyloid beta deposition. No complaints are mentioned.
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Review of: A-beta, BioLegend, 803001 by Swagata Ghatak, The Scripps Research Institute
Works fine for ICC of hiPSC derived cerebral organoids.
Dilution used: 1:2000
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1
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0 not usefulReview of: A-beta, BioLegend, 803001 by Maarten Rotman
I used mouse IgG1 anti human Abeta1-16 (Covance, clone 6E10, # SIG-39320 ) to detect amyloid beta depositions. [note: Covance antibodies has now become BioLegend]
Conditions: 1:500 dilution of antibody in 1% BSA in PBS solution, fixative was ice cold acetone, block solution was DAKO peroxidase blocking reagent.
Tissues: human post mortem cryosections of the cortex of healthy controls, and human AD/CAA and human HCHWA-D patients.
Used DAKO EnVision anti mouse polymer-HRP as a secondary ‘antibody’ treatment.
Results: worked beautifully (just as well as m-a-Abeta clone 4G8, a more regularly used antibody for ABeta detection)
For more info see publication 10.1016/j.neuroscience.2011.05.076 or AlzForum post http://www.alzforum.org/antibodies/app-or-v-amyloid-6e10-0
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