A-beta, BioLegend, 803001

Works fine for ICC of hiPSC derived cerebral organoids.

Dilution used: 1:2000

For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1

I used mouse IgG1 anti human Abeta1-16 (Covance, clone 6E10, # SIG-39320 ) to detect amyloid beta depositions. [note: Covance antibodies has now become BioLegend]

Conditions: 1:500 dilution of antibody in 1% BSA in PBS solution, fixative was ice cold acetone, block solution was DAKO peroxidase blocking reagent.

Tissues: human post mortem cryosections of the cortex of healthy controls, and human AD/CAA and human HCHWA-D patients.

Used DAKO EnVision anti mouse polymer-HRP as a secondary ‘antibody’ treatment.

Results: worked beautifully (just as well as m-a-Abeta clone 4G8, a more regularly used antibody for ABeta detection)

For more info see publication 10.1016/j.neuroscience.2011.05.076 or AlzForum post http://www.alzforum.org/antibodies/app-or-v-amyloid-6e10-0