Myc epitope tag, Cell Signaling Technology, 2278
What do reviewers generally think about 2278 from Cell Signaling Technology?
Researchers agree that the antibody works for immunofluorescence to detect Myc-tagged proteins, with successful use in breast cancer cells and HEK293 cells. One reviewer points out that the signal-to-noise is not very high, although transfected and untransfected cells remain distinguishable, and recommends a 1:200 dilution in 10% FBS.
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User Reviews (2)
Review of: Myc epitope tag, Cell Signaling Technology, 2278 by Michael J. Lukey, Cold Spring Harbor Laboratory
Fine antibody for IF. Detects Myc-tagged proteins in breast cancer cells.
For more information see Lukey et al. Cell Reports 2019, “Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer”.
https://www.sciencedirect.com/science/article/pii/S2211124719311313#mmc1
Review of: Myc epitope tag, Cell Signaling Technology, 2278 by Peter Lund Ovesen, Aarhus University
Works in IF to detect Myc-tagged proteins in Hek293 cells. Not very high signal to noise, but you can see the difference between transfected and un-transfected cells.
Dilution: 1:200 in 10 % FBS
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