Shank3, Synaptic Systems, 162 304
What do reviewers generally think about 162 304 from Synaptic Systems?
Researchers report that Shank3, Synaptic Systems, 162 304 performs well for immunocytochemistry in primary neuronal cultures, detecting postsynaptic-density-like structures along dendrites of mature hippocampal neurons. Both used a 1:500 dilution after PFA fixation, with one using overnight 4°C incubation and the other 10% FBS blocking. One reviewer points out that the antibody works very nicely for ICC in rat primary neuronal cultures with PFA fixation and overnight 4°C incubation.
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Review of: Shank3, Synaptic Systems, 162 304 by Julia Bär
Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
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0 not usefulReview of: Shank3, Synaptic Systems, 162 304 by Peter Lund Ovesen, Aarhus University
Shank3 guinea pig polyclonal, antiserum (162 304) from Synaptic Systems
This antibody detects postsynaptic-density-like structures along the dendrites of mature primary hippocampal neurons (DIV14).
Neurons were fixed in 4% PFA, permabilized in 0,1 % Triton-X100 and blocked in 10 % FBS
Antibody dilution: 1:500 in 10 % FBS
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