GFP, Abcam, ab13970
What do reviewers generally think about ab13970 from Abcam?
Researchers consistently report that GFP, Abcam, ab13970 performs well for immunofluorescence and immunostaining, including ICC on fly brains and labella and perfusion-fixed mouse brain or tissue sections. It is described as specific, robust, and usable at relatively dilute concentrations, with reported dilutions from 1:500 to 1:10,000. One reviewer points out that they have used this antibody for many years across almost eight different batches without complaints, noting consistent batch-to-batch performance. No major complaints are mentioned.
Information about this antibody
Vendor notes
User Reviews (8)
Review of: GFP, Abcam, ab13970 by Bing Zhang initiative
Works great for ICC on fly brains and labella. Dilution: 1:1000.
0 useful
0 not usefulReview of: GFP, Abcam, ab13970 by Xi Zha
This antibody worked great for immunostaining of perfusion-fixed mice brain slices for IF.
Dilution used: 1:1000
For more information see Zha et al. Cell Reports 2020, “VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression”.
https://pubmed.ncbi.nlm.nih.gov/32320666/
Comments
0 useful 0 not usefulReview of: GFP, Abcam, ab13970 by Jimena Berni, University of Cambridge
Great antibody for immunostaining.
Dilution used: 1:2000
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0 useful 0 not usefulReview of: GFP, Abcam, ab13970 by Justin Botterill, The Nathan S. Kline Institute for Psychiatric Research
Immunostaining were done on 4% paraformaldehyde perfusion-fixed brains that were cut at 50µm on a vibratome.
Antibody specificity is good and results in robust labeling. Can use relatively dilute concentrations, depending on signal (e.g., 1:2,000 to 1:10,000)
For more information see Botterill et al. Cell Reports 2019, “An Excitatory and Epileptogenic Effect of Dentate Gyrus Mossy Cells in a Mouse Model of Epilepsy”
https://www.sciencedirect.com/science/article/pii/S2211124719314330
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0 useful 0 not usefulReview of: GFP, Abcam, ab13970 by Huda Zoghbi, Baylor College of Medicine
Great antibody for IF.
Dilution used: 1:800
For more information see Lavery et al. eLife 2020, “ Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
https://elifesciences.org/articles/52981
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0 useful 0 not usefulReview of: GFP, Abcam, ab13970 by Wei Li, University of Chinese Academy of Sciences, Beijing
This antibody worked great for immunostaining.
Dilution used: 1:500
For more information see Cui et al. Cell Reports 2019, “Derivation of Mouse Haploid Trophoblast Stem Cells”.
https://www.sciencedirect.com/science/article/pii/S2211124718320126#
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0 useful 0 not usefulReview of: GFP, Abcam, ab13970 by David I. Hughes, University of Glasgow
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:1000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes
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0 useful 0 not usefulReview of: GFP, Abcam, ab13970 by Sulieman Minhas
Anti-GFP Chicken antibody ab13970 Abcam
We have been using this particular antibody for many years and almost 8 different batches. for immunostainings, never had any complaints and all the batches work the same.
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0 useful 0 not useful
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