Syndecan-1, Abcam, ab181789
What do reviewers generally think about ab181789 from Abcam?
Researchers report mixed results using this antibody for Western blotting of HepG2 lysates at 1:1000. One reviewer points out that a clear band at approximately 62 kDa was observed, with only weak extra bands after prolonged exposure. Another reviewer found many clearly separated bands, making it difficult to identify the correct band, contrary to the expected single band. Overall, performance appears inconsistent, with useful detection in one case but poor specificity in another.
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User Reviews (2)
Review of: Syndecan-1, Abcam, ab181789 by Julie Torvund-Jensen
Anti-syndecan1 Mouse monoclonal [1A3H4] abcam ab181789
I have used this antibody for Western blots of HepG2 lysates with nice results. A clear band at approx. 62 kDa is observed. A couple of othe bands can also be seen, however, these are very weak and only visible after prolonged exposure of the blot.
I have used a 1:1000 dilution.
0 useful
0 not usefulReview of: Syndecan-1, Abcam, ab181789 by Ditte Olsen, Aarhus University
Anti-syndecan1 Mouse monoclonal [1A3H4] abcam ab181789
We have tested this antibody on HepG2 cell lysate in Western Blotting. According to abcam, a clear single band should be expected when using this cell line. This was not the case for us. Many clearly separated bands were observed. This makes it very difficult to know which band/bands is/are correct.
The antibody was diluted 1:1000
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