LDLR, Abcam, 175883
What do reviewers generally think about 175883 from Abcam?
Researchers report poor performance of LDLR, Abcam, 175883. One reviewer points out that Abcam retests found no bands in mouse lung, liver, colon or human liver, only high-background bands in HepG2 and HeLa, leading to withdrawal for lack of LDLR specificity. Another used it at 1:1000 for Western blot of endogenous LDLR in PCSK9-stimulated HepG2 cells and saw faint bands of expected size, but it failed to detect the PCSK9-induced LDLR reduction. Overall, the consensus is that the antibody is unreliable for detecting LDLR in these applications.
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User Reviews (2)
Review of: LDLR, Abcam, 175883 by Camilla Gustafsen, Aarhus University
We have received an e-mail from Abcam with the following information regarding anti-LDLR antibody ab175883:
“Recent retests of ab175883 in western blot (WB) on mouse: lung, liver, colon and on human: liver showed negative results (i.e. no band). A band at the correct molecular weight was detected in HepG2 and HeLa cell lines but with high background. Therefore we have concluded that ab175883 is not specific to LDL Receptor and we have now withdrawn it from sale”
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0 not usefulReview of: LDLR, Abcam, 175883 by Ditte Olsen, Aarhus University
Anti-LDLR Rabbit polyclonal abtibody, abcam175883
I have used this antibody diluted 1:1000 for WB detection of endogenous LDLR in HepG2 cells after stimulation with PCSK9. Although faint bands were observed of expected size, the antibody failed to detect PCSK9 induced reduction of LDLR.
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