C-Myc, Absolute Antibody, Ab00100-1.1
What do reviewers generally think about Ab00100-1.1 from Absolute Antibody?
Researchers report C-Myc Absolute Antibody Ab00100-1.1 performs well for immunoprecipitation of myc-tagged purified proteins at 0.5 µg/sample and for western blotting at 1:1000, detecting purified or 1 µg myc-tagged protein with a strong band and untagged protein as negative control. One reviewer points out that the antibody may be diluted further and that less protein could be loaded. No complaints are mentioned.
Information about this antibody
Vendor notes
User Reviews (3)
Review of: C-Myc, Absolute Antibody, Ab00100-1.1 by Sune Skeldal, Aarhus University
Some success with this antibody in regards to IP of myc-tagged purified proteins (0.5 µg/sample).
0 useful
0 not usefulReview of: C-Myc, Absolute Antibody, Ab00100-1.1 by Sune Skeldal, Aarhus University
Works well in western blotting (1:1000) for detection of purified protein.
Comments
0 useful 0 not usefulReview of: C-Myc, Absolute Antibody, Ab00100-1.1 by Camilla Gustafsen, Aarhus University
Myc epitope tag
clone 9E10
mAb, mouse IgG1
Absolute Antibody
Ab00100-1.1
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded
2. Ab: HRP-anti Mouse IgG, Cell Signaling 7076S (1:2000), 1 hour at RT
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0 useful 0 not useful
Comments