Myc epitope tag, Absolute Antibody, Ab00100-23.0
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Review of: Myc epitope tag, Absolute Antibody, Ab00100-23.0 by Anders Dalby, Aarhus University
Antibody: anti-myc epitope tag Rabbit IGG Ab00100-23.0 [9E10] (Absolute antibody)
The antibody was used to visualize myc-tagged proteins in transfected primary hippocampal neurons. Due to a high background signal, we were unable to distinguish the negative control from positive.
A concentration of 2ug/mL was used.
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0 not usefulReview of: Myc epitope tag, Absolute Antibody, Ab00100-23.0 by Camilla Gustafsen, Aarhus University
Myc epitope tag
clone 9E10
mAb, rabbit IgG
Absolute Antibody
Ab00100-23.0
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded.
2. Ab: HRP-anti Rabbit IgG, Cell Signaling 7074S (1:2000), 1 hour at RT
Similar band-intensity was seen with anti-Myc tag antibodies Ab00100-1.1 and Ab00100-2.0
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