N-WASP, Atlas Antibodies AB, HPA005750
What do reviewers generally think about HPA005750 from Atlas Antibodies AB?
Researchers report the antibody works for Western blotting on mouse brain, HEK, and pig brain lysates, detecting a band near the expected 70 kDa, though with additional weaker or intense bands in the 25-75 kDa range. One reviewer points out that the recommended 1/500 dilution can overexpose bands and suggests using a higher dilution, such as 1/5000, which detected the appropriate band with weaker extra bands.
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User Reviews (2)
Review of: N-WASP, Atlas Antibodies AB, HPA005750 by Veerle Paternoster
Tested on whole brain cell lyaste from mouse. Loaded: 30 ug and 10 ug, WB using stainfree gel/membrane, semi-dry blotting, ECL detection.
Detects a band of appropriate size, together with some weaker bands.
Dilution: 1/5000.
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0 not usefulReview of: N-WASP, Atlas Antibodies AB, HPA005750 by Veerle Paternoster
Tested for Western blotting on 100 ug total cell lysate from HEK cells, mouse brain and pig brain.
Gives several intense bands between 25-75 kDa . Isolated band around the expected size (70 kDa) in all extracts.
used dilution: 1/500 (recommended), but overexposed bands. I would highly recommend to dilute more.
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