Catenin, BD Bioscience, 610133
What do reviewers generally think about 610133 from BD Bioscience?
Researchers report that this mouse monoclonal catenin antibody performs well for immunofluorescence in brain endothelial cells, giving clear, strong signal in mouse, human, and primary porcine cells at 1:100 to 1:200 dilution with 4% paraformaldehyde or methanol fixation. One reviewer points out that a goat anti-mouse Alexa Fluor 488 secondary at 1:350, with Triton-X100 permeabilization and BSA blocking, produced good staining. No complaints are mentioned.
Information about this antibody
Vendor notes
User Reviews (2)
Review of: Catenin, BD Bioscience, 610133 by Andrea E. Toth, Aarhus University
This antibody works very well for immunofluorescence staining in mouse (b.End3) and human (hCMEC/D3) brain endothelial cell line. Dilution 1:200. Fixation was done both with 4% paraformaldehyde or methanol.
0 useful
0 not usefulReview of: Catenin, BD Bioscience, 610133 by Piotr Siupka, Aarhus University
Producer: BD Transduction Laboratories
Catalog No: 610133
Used dilution: 1:100 (IF)
Cell line: primary porcine brain endothelial cells
Host organism: Mouse
Character: primary, monoclonal
Antibody working very good, giving clear strong signal in primary porcine brain endothelial cells at indicated dilution. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 488 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
Comments
0 useful 0 not useful
Comments