ZO-1, Thermo Fischer Scientific, 61-7300
What do reviewers generally think about 61-7300 from Thermo Fisher Scientific?
Researchers report that the ZO-1 antibody performs well for immunofluorescence and immunohistochemistry. It worked at 1:100 for IF in primary porcine brain endothelial cells and for immunostaining, and at 1:400 overnight for IHC on PFA-fixed, paraffin-embedded human breast cancer tissue, giving specific tight junction punctate labeling in ducts. One reviewer points out that target retrieval was performed in TEG buffer and DAB development was used for 1 min 15 sec. The reviews do not mention failures.
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Vendor notes
User Reviews (3)
Review of: ZO-1, Thermo Fischer Scientific, 61-7300 by Wei Li, University of Chinese Academy of Sciences, Beijing
This antibody worked fine for immunostaining.
Dilution used: 1:100
For more information see Cui et al. Cell Reports 2019, “Derivation of Mouse Haploid Trophoblast Stem Cells”.
https://www.sciencedirect.com/science/article/pii/S2211124718320126#
0 useful
0 not usefulReview of: ZO-1, Thermo Fischer Scientific, 61-7300 by Helene H. Jensen, Aarhus University
ZO-1 (zonula occludens 1)
Thermo, 61-7300
We used this antibody for IHC-labeling of human breast cancer tissue. The antibody gave a specific labeling of classical tight juntion “punctae” in ducts.
The antibody was used at 1:400 ON. The tissue was PFA-fixed, embedded in paraffin, cut at 3 um, and target retrival was performed in TEG-buffer (Tris/EGTA). The secondary antibody was used at 1:200 and developed with DAB for 1 min 15 sec.
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0 useful 0 not usefulReview of: TJP1/ZO-1, Thermo Fischer Scientific, 617 300 by Piotr Siupka, Aarhus University
Producer: Invitrogen
Catalog No: 617 300
Used dilution: 1:100 (IF)
Cell line: primary porcine brain endothelial cells
Host organism: Rabbit
Character: primary, polyclonal
Antibody working nice at indicated dilution. Tested in primary porcine brain endothelial cells. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
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