ZO-1, Thermo Fischer Scientific, 61-7300
Information about this antibody
Vendor notes
User Reviews (3)
Review of: ZO-1, Thermo Fischer Scientific, 61-7300 by Wei Li, University of Chinese Academy of Sciences, Beijing
This antibody worked fine for immunostaining.
Dilution used: 1:100
For more information see Cui et al. Cell Reports 2019, “Derivation of Mouse Haploid Trophoblast Stem Cells”.
https://www.sciencedirect.com/science/article/pii/S2211124718320126#
0 useful
0 not usefulReview of: ZO-1, Thermo Fischer Scientific, 61-7300 by Helene H. Jensen, Aarhus University
ZO-1 (zonula occludens 1)
Thermo, 61-7300
We used this antibody for IHC-labeling of human breast cancer tissue. The antibody gave a specific labeling of classical tight juntion “punctae” in ducts.
The antibody was used at 1:400 ON. The tissue was PFA-fixed, embedded in paraffin, cut at 3 um, and target retrival was performed in TEG-buffer (Tris/EGTA). The secondary antibody was used at 1:200 and developed with DAB for 1 min 15 sec.
Comments
0 useful 0 not usefulReview of: TJP1/ZO-1, Thermo Fischer Scientific, 617 300 by Piotr Siupka, Aarhus University
Producer: Invitrogen
Catalog No: 617 300
Used dilution: 1:100 (IF)
Cell line: primary porcine brain endothelial cells
Host organism: Rabbit
Character: primary, polyclonal
Antibody working nice at indicated dilution. Tested in primary porcine brain endothelial cells. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
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