CI-M6PR/IGF2R, Developmental Studies Hybridome Bank, AB_528348

Producer: Developmental Studies Hybridoma Bank
Catalog No: 86f7
Used dilution: 1:100; 1:200; 1:300 (IF); 1:100 (WB)
Cell line: primary bovine brain endothelial cells, primary porcine brain endothelial cells
Host organism: Ms
Character: primary, monoclonal

Antibody tested for IF and WB. Raised against bovine CI-M6PR works nicely in primary bovine brain endothelial cells in mentioned dilutions (most often 1:100 used). Both WB and IF looks good.
For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 488 (1:350) was used.

Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.