BirA, Abcam, ab14002
What do reviewers generally think about ab14002 from Abcam?
Researchers using Abcam ab14002 chicken polyclonal anti-BirA report poor performance in Western blot and fixed-cell staining on HEK cells expressing BirA fusion proteins, with nonspecific bands or high background, including in untransfected controls. One reviewer points out that Abcam appears to have discontinued the antibody, suggesting the company reached a similar conclusion. Another notes specific staining could be distinguished from background in HEK293 and RN22 cells, but overall staining was unimpressive. The consensus is that the antibody is not recommended for these applications.
User Reviews (3)
Review of: BirA, Abcam, ab14002 by Arulmani Manavalan
I also tried Anti-BirA (Abcam’s ab14002) polyclonal Chicken western (1:1000) using HEK cell lysates overexpressing a BirA fusion construct (After Transmembrane domain). observed Unspecific bands around 129, 75, 50 and 45 kDa including Control Hek lysate (Un-transfected)
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0 not usefulReview of: BirA, Abcam, ab14002 by Maarten Rotman
Used Abcam’s ab14002 polyclonal Chicken-anti-BirA on WB using HEK cell lysates overexpressing a BirA fusion construct, but I can’t recommend that. Lot’s of a-specific staining. I also saw that Abcam discontinued this antibody, I guess they came to the same conclusion.
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0 useful 0 not usefulReview of: BirA, Abcam, ab14002 by Maarten Rotman, Mayo clinic, Jacksonville
I’ve used Abcam’s ab14002 polyclonal Chicken-anti-BirA at 5.5 µg/ml in blockbuffer (1%BSA in PBS) on transiently transfected, 4% PFA fixed, 1% triton X100 permeabilized HEK293 and RN22 cells (type 1 transmembrane fusion protein w/ the BirA just extracellular from the membrane). Secondary was LifeTech’s Goat-anti-Chicken Alexa 488. Primary staining o/n at 4C, secondary 1 h at RT.
The staining suffered high a-specific background in HEK cells and less but definitely still present background in the RN22 cells. It was clearly possible to distinguish specific staining from background, but I was not too impressed with the staining in general.
Didn’t test it in any other modality, concentration, or protocol yet.
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