Histone H3, Abcam, ab1791
What do reviewers generally think about ab1791 from Abcam?
Researchers generally report that Histone H3, Abcam, ab1791 performs well for Western blotting, often at 1:20,000, including with histone extracts and RIPA lysates, and one reviewer points out that its C-terminal epitope allows detection of clipped histone H3. It was also used for immunocytochemistry on fly embryos at 1:1000 and co-IP with 1 ug. A specific complaint is that it did not work for immunostaining of Drosophila imaginal discs.
Information about this antibody
Vendor notes
User Reviews (4)
Review of: Histone H3, Abcam, ab1791 by Bing Zhang initiative
Used for ICC on fly embryo at the dilution of 1:1000.
1ug used for CO-IP.
Used for WB at the dilution of 1:20.000.
0 useful
0 not usefulReview of: Histone H3, Abcam, ab1791 by Huda Zoghbi, Baylor College of Medicine
Worked fine for WB.
Dilution used: 1:20.000
For more information see Lavery et al. eLife 2020, “ Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
https://elifesciences.org/articles/52981
Comments
0 useful 0 not usefulReview of: Histone H3, Abcam, ab1791 by Veerle Paternoster
Histone H3, Rabbit, Abcam #ab1791
Excellent antibody for Western blotting. Works both on histone extracts (acid extraction, mouse brain) as cell lysates (RIPA buffer, mouse brain).
C-terminal epitope, so can detect clipped histone H3 as well.
WB details: BIS-TRIS gel+XT-MES running buffer, blocked in TBS+5%BSA, 1:20 000 dilution.
Comments
0 useful 0 not usefulReview of: Histone H3, Abcam, ab1791 by Philip Yung, Cavalli lab, CNRS
Not working for immunostaining of Drosophila Imaginal Discs
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0 useful 0 not useful
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