Aggrecan, merckmillipore, MM_NF-AB1031
What do reviewers generally think about MM_NF-AB1031 from Sigma-Aldrich?
Researchers view the antibody favorably for detecting aggrecan/chondroitin sulfate proteoglycan in perineuronal nets and for IHC, with one citing stroke-related labeling changes. It also works well in Western blot. One reviewer points out that using a 1:1000 dilution, 1% BSA blocking, and 4-8% Tris-Acetate gels with the corresponding buffer gives good Western blot results. No complaints are mentioned.
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User Reviews (2)
Review of: Aggrecan, merckmillipore, MM_NF-AB1031 by Wolfgang Härtig, University of Leipzig
Rabbit-anti-aggrecan (Merck Millipore; AB1031) detects the main chondroitin sulphate proteoglycan (CSPG) in perineuronal nets (PNs) of the extracellular matrix. PNs are lattice-like coatings around certain neurons in the nervous system of various animal species. They are composed of hyaluronic acid, CSPGs and tenascins, and their complexity allows for numerous detection methods, but AB1031 is among their favoured markers. Altered labelling of PNs after experimentally induced stroke was reported (Härtig et al 2016 Neuroscience – Fig 6; Härtig et al 2017 Front Integr Neurosci – Figs 7,8).
I can highly recommend this antibody for IHC
Härtig et al. 2016 Neuroscience: https://www.ncbi.nlm.nih.gov/pubmed/27634771
Härtig et al. 2017 Front Integr Neurosci: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559442/
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0 not usefulReview of: Aggrecan, merckmillipore, MM_NF-AB1031 by Julia Abele, University of Magdeburg
dilution 1:1000, block with 1% BSA, use 4-8% Tris-Acetat Gels and corresponding buffer system. anti-Aggrecan antibody works nice in Western Blot
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