Phospho-GSK-3β, Cell Signaling Technology, 9336
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User Reviews (3)
Review of: Phospho-GSK-3β, Cell Signaling Technology, 9336 by Giorgio Ramadori, University of Geneva, Department of Cellular Physiology and Metabolism
We have used this antibody for western blotting with great succes.
For more information see Aras et al., 2019, Cell Reports 27, 2385–2398 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30573-X)
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0 not usefulReview of: Phospho-GSK-3β, Cell Signaling Technology, 9336 by Marko Rosenholm
Phospho-GSK-3β (Ser9) Antibody #9336
Cell Signaling Technology
This antibody has regularly given a clear and specific band at expected molecular weight when detecting GSK3β phosphorylation in mouse hippocampal and cortical lysates in Western blot.
The antibody is diluted 1:1000 in 3% BSA in TBS-T + 1:1000 NaN3. Dilution has given a good signal for 5-7 uses when stored at 4 degrees.
Incubation overnight at 4 degrees
Washing steps with TBS-T
Secondary antibody diluted in 5% milk powder in TBS-T for 1 hour
Detection with ECL+ (ThermoScientific)
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0 useful 0 not usefulReview of: Phospho-GSK-3β, Cell Signaling Technology, 9336 by Mads Kjolby, Aarhus University
I have used anti-phosphoGK3beta Ser9 (CST #9336) at 1:1000 on murine heart tissue. Gives a nice band at 45 kDa as expected.
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