GAPDH, Novus Biologicals, NB300-221
Information about this antibody
Target
Alt. Targets
GAPDH
Vendor
Catalog No
NB300-221
Application
Clonality
mAb
Origin
Mouse
Reactivity
Hamster, Cat, Zebrafish, Chicken, Rabbit, Chinese Hamster
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: GAPDH, Novus Biologicals, NB300-221 by Juan Yuan, Aarhus University
This antibody is excellent to be chosen as an internal control for western blot. I have tested both in neural progenitor cell and medulloblastoma cell. Both cell line the signal is very clear, sharp, specific, no background.
Primary antibody: 1:1000 dilution in 5%milk/TBST buffer, incubate in RT for 1 hour.
Secondary antibody: anti-mouse IgG-HRP, 1:10000 dilution in 5%milk/TBST buffer, incubate inRT for 1 hour.
Image the signal using ImageQuant for exposure time of 1-3 sec.
See image above.
0 useful
0 not useful
Comments