GFAP, Sigma-Aldrich, G3893
What do reviewers generally think about G3893 from Sigma-Aldrich?
Researchers report that GFAP, Sigma-Aldrich, G3893 performs well in immunohistochemistry, immunofluorescence, and western blotting on mouse and rat tissue, using 1:1000 for IHC/IF and 1:7000 for western blotting. One reviewer points out that a 24-hour incubation at 4°C is optimal for 30 µm mouse brain sections. No adverse performance issues are mentioned.
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User Reviews (2)
Review of: GFAP, Sigma-Aldrich, G3893 by Shirin Hosseini, Technische Universität Braunschweig
I have used this antibody for immunohistochemistry of 30 µm brain sections of C57BL/6J mice in a dilution of 1:1000. The optimum incubation time for this antibody is 24 hour at 4◦C. This antibody works so well in our lab.
For more information see Hosseini et al. Cell Reports 2020, “Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS”.
https://www.sciencedirect.com/science/article/pii/S2211124720306197
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0 not usefulReview of: GFAP, Sigma-Aldrich, G3893 by Kalpana Shrivastava, Aarhus University
Antibody: GFAP mouse monoclonal; dilution: 1:1,000 for Immunofluorescence and 1:7,000 for western blotting, catalogue no.: G3893 Sigma Aldrich.
Antibody works good in both mouse and rat tissue for immunohistochemistry as well as western blotting. Blocking buffer used for IHC: 0.3%BSA with 10% FCS in TBS with 0.5% triton and for Western blotting: BB [5% nonfat dry milk (sc-2325, Santa Cruz Biotechnology), in TBS + 0.1% Tween 20 (93773, Sigma)]
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