Parvalbumin, merckmillipore, MAB1572
What do reviewers generally think about MAB1572 from Sigma-Aldrich?
Researchers report mixed performance for Parvalbumin MAB1572. It works extremely well for immunofluorescence and DAB staining in mouse and human fixed brain sections, with high signal and low noise, and yields a single Western blot band at the expected size. One reviewer points out that the low molecular weight of the protein made Western blotting inherently tricky. Another reviewer found it did not work very well for immunocytochemistry of hiPSC-derived neuronal cultures at 1:100. Overall, it is strong for fixed brain section applications but less reliable for some neuronal culture ICC.
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User Reviews (2)
Review of: Parvalbumin, merckmillipore, MAB1572 by Swagata Ghatak, The Scripps Research Institute
Did not work very well for ICC of hiPSC derived neuronal cultures.
Dilution used: 1:100
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1
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0 not usefulReview of: Parvalbumin, merckmillipore, MAB1572 by Elizabeth Lucas, Mount Sinai Hospital
Protocols used: DAB (1:2000), immunofluorescence (1:500 – 1:1,1000), Western blot (1:500)
Species: mouse, human
This antibody works extremely well for immunofluorescence and DAB staining in mouse and human fixed brain sections, giving high signal and low noise for both species. For Western blot, this antibody gives a single band at the specified molecular weight, but the low weight of this protein made the protocol inherently tricky.
References:
Lucas, E.K., Reid, C.S., McMeekin, L.J., Dougherty, S.E., & Cowell, R.M. (2015). Cerebellar transcriptional alterations with Purkinje cell dysfunction and loss in mice lacking PGC-1α. Frontiers in Cellular Neuroscience, Special Issue, Neurodegeneration: from Genetics to Molecules, 8, 441:1-13.
Lucas, E.K., Dougherty, S.E., McMeekin, L.J., Reid, C.S., West, A.B., Dobrunz, L.E., Hablitz, J.J., & Cowell, R.M. (2014). PGC-1α provides a transcriptional framework for synchronous neurotransmitter release from parvalbumin-positive interneurons. Journal of Neuroscience, 34, 43: 14375-14387.
Lucas, E.K., Jegarl, A., & Clem, R.L. (2014). Mice lacking TrkB in parvalbumin-positive cells exhibit sexually dimorphic behavioral phenotypes. Behavioural Brain Research, 274: 219-225.
Dougherty, S.E., Reeves, J. L., Lucas, E. K., Lesort, M. J., Detloff, P. J. Gamble, K. L., & Cowell, R. M. (2012). Presymptomatic abnormalities in Purkinje cell function and viability in animal models of HD. Experimental Neurology, 236: 171-178.
Lucas, E.K., Markwardt, S.J., Gupta, S., Meador-Woodruff, J.H., Lin, J.D., Overstreet-Wadiche, L., & Cowell, R.M. (2010). Parvalbumin deficiency and GABAergic dysfunction in mice lacking PGC-1α. Journal of Neuroscience, 30, 21, 7227-7235.
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