GABA A receptor alpha 2, Synaptic Systems, 224 103

This antibody also works perfectly for ICC. Staining on hippocampal neurons showed a clear staining which co-localized with gephyrin, a marker of inhibitory synapses.

Used at a dilution of 1:500.

This antibody was tested on 15 um thick hippocampal cryo sections from a perfusion fixed mouse. Prior to immunohistochemistry, the tissue was target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.

This antibody works excellent for detecting expression of GABA(A) receptor subtype alpha 2 in the hippocampus.

This was tested at 1:250, 1:500 and 1:1000. At 1:1000 a clear signal was seen with very low background.