GABA A receptor alpha 2, Synaptic Systems, 224 103
What do reviewers generally think about 224 103 from Synaptic Systems?
Researchers report the antibody performs well for immunocytochemistry and immunohistochemistry on hippocampal neurons and cryosections, giving clear staining that co-localizes with gephyrin and detects GABA A receptor alpha 2 with low background. Tested dilutions include 1:250, 1:500, and 1:1000; One reviewer points out that at 1:1000 a clear signal was seen with very low background. No complaints are mentioned.
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Review of: GABA A receptor alpha 2, Synaptic Systems, 224 103 by Simon Mølgaard, Aarhus University
This antibody also works perfectly for ICC. Staining on hippocampal neurons showed a clear staining which co-localized with gephyrin, a marker of inhibitory synapses.
Used at a dilution of 1:500.
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0 not usefulReview of: GABA A receptor alpha 2, Synaptic Systems, 224 103 by Simon Mølgaard, Aarhus University
This antibody was tested on 15 um thick hippocampal cryo sections from a perfusion fixed mouse. Prior to immunohistochemistry, the tissue was target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.
This antibody works excellent for detecting expression of GABA(A) receptor subtype alpha 2 in the hippocampus.
This was tested at 1:250, 1:500 and 1:1000. At 1:1000 a clear signal was seen with very low background.
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